From owner-drosophila@net.bio.net Mon May 27 23:00:00 1996
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From: adhar@leland.Stanford.EDU (Adam Noel Harris)
Newsgroups: bionet.drosophila
Subject: Re: Flip-Out
Date: 28 May 1996 10:44:40 -0700
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Pineda (pinema00@ac.usfca.edu) said this stuff:

: 	Our lab is looking to induce mutations through the use of
: transposons.  We have heard of a system which involved a stock
: with a transposable element (without transopsase) and another stock containing
: a transposase.  Any ideas?

I did a literature search on subject "drosophila p element mutagenesis"
and got 43 citations.  Try:

Cooley, L., Kelley, R., and Spradling, A. 1988.  Insertional mutagenesis
of the Drosophila genome with single P elements.
Science 239(4844):1121-28.

We commonly use delta 2-3 for the transposase source.

Was there specific information which you wanted?

-Adam
 

.