From owner-biophys@hgmp.mrc.ac.uk Thu Apr 22 02:50:05 2004 Return-Path: X-Original-To: biophys-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 504BC7D2D9 for ; Thu, 22 Apr 2004 02:50:05 +0100 (BST) X-Original-To: biophys-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B5D237D2DC for ; Thu, 22 Apr 2004 02:50:04 +0100 (BST) Received: from helium.hgmp.mrc.ac.uk (helium [193.62.192.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id EB5797D2EB for ; Thu, 22 Apr 2004 02:50:02 +0100 (BST) Received: (from news@localhost) by helium.hgmp.mrc.ac.uk (8.12.10+Sun/8.12.1/Submit) id i3M1o21j014929 for biophys-list@hgmp.mrc.ac.uk; Thu, 22 Apr 2004 02:50:02 +0100 (BST) From: Bhadresh Rami X-Newsgroups: bionet.biophysics Subject: immobilized pepsin columns Date: Wed, 21 Apr 2004 18:49:55 -0700 Lines: 15 Message-ID: References: <200404201940.i3KJewo18096@vreme.yubc.net> Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Complaints-To: news@news.stanford.edu In-Reply-To: <200404201940.i3KJewo18096@vreme.yubc.net> To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk dear friends, does anyone have experience with immobilized pepsin columns?? i need one to perform online digestion (pH 2.5, 0 C) followed by feeding into the massspec for fragment analysis. any input on the brand, quality, comparative efficiencies of these columns will be very helpful... thanks. - rami .