From owner-celegans@net.bio.net Sun Jul 13 23:00:00 1997 Path: biosci!agate!spool.mu.edu!sol.net!spool.mu.edu!newsspool.sol.net!howland.erols.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-xfer.netaxs.com!netnews.upenn.edu!NewsWatcher!user From: rappaport@email.chop.edu (Eric Rappaport) Newsgroups: bionet.celegans Subject: DNA SEQUENCERS FOR SALE Date: Mon, 14 Jul 1997 15:10:48 -0400 Organization: Children's Hospital of Philadelphia Lines: 64 Message-ID: NNTP-Posting-Host: 159.14.43.32 AUTOMATED DNA SEQUENCERS FOR SALE Three ABI 373 DNA sequencers, all currently in use and under service contract are available for sale. Please contact Dr. Eric Rappaport (rappaport@email.chop.edu) with offers or questions. Details of the equipment configuration follow: Two 373 units with Stretch upgrades: Filters - 5-filter wheel (can choose between 2 different sets of 4 different dyes). Chemistries - Standard Taq (FS) terminator, dye primer or ABI T7 polymerase sequencing chemistry. All current Genescan chemistries, including microsatellite panels, primers labeled during synthesis with standard ABI dyes (6-Fam, Hex, Tet, Tamra) or (6-Fam, Hex, Tamra, and Rox). Gel lengths - Multiple gel (well-to-read) lengths including 48 cm (standard long-read [>650 nt] sequencing length), 34 (shorter sequence reads [550-600 nt] or high-resolution Genescan applications), 24 (microsatellite applications) 12 (lower-resolution Genescan applications) or 6 (lower-resolution Genescan applications). Scan Speed - 1X, BaseSprinter (2X, but limitedto middle half of gel; can be used for faster analysis of shorter sequences). One 373 with multiple gel length (Leon) configuration: Filters - 5-filter wheel (can choose between 2 different sets of 4 different dyes). Chemistries - Standard Taq (FS) terminator, dye primer or ABI T7 polymerase sequencing chemistry. All current Genescan chemistries, including microsatellite panels, primers labeled during synthesis with standard ABI dyes (6-Fam, Hex, Tet, Tamra) or (6-Fam, Hex, Tamra, and Rox). Gel lengths - Multiple gel (well-to-read) lengths including 24 cm (shorter sequence reads [400-500 nt] or microsatellite applications) 12 (lower-resolution Genescan applications) or 6 (lower-resolution Genescan applications). Scan Speed - 1X, BaseSprinter (2X, but limitedto middle half of gel; can be used for faster analysis of shorter sequences). Computers and Software Unit 1 (Stretch) - Mac IIcx with 8 mB RAM/230 mB hard drive Unit 2 (Stretch) - Mac IIci with 8 mB RAM/100 mB hard drive Unit 3 (Leon) - Mac IIci with 8 mB RAM/120 mB hard drive All units are equipped with latest versions of analysis and collection sofware for running sequencing and Genescan applications on the above computers. Upgrades for PowerMac are not included. Service Contracts All units have been under service contract since the time of their purchase from ABI and are currently covered under those agreements. Purchaser would need to have an installation service call from ABI before initiation of a new service contract could occur. .