From owner-dros@hgmp.mrc.ac.uk Fri Nov 17 19:03:15 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 41A4E17ABB; Fri, 17 Nov 2000 19:03:14 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 074F917AA5; Fri, 17 Nov 2000 19:03:12 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id E80A617B41; Fri, 17 Nov 2000 14:53:53 +0000 (GMT) To: bionet-drosophila@net.bio.net From: cnm5@leicester.ac.uk ("Moran, C.N.") Newsgroups: bionet.drosophila Subject: RNA in situs Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: MIME-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 0B54117B40; Fri, 17 Nov 2000 14:53:50 +0000 (GMT) X-Received: from artemis.le.ac.uk (artemis.le.ac.uk [143.210.16.126]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id BACA8415CF for ; Fri, 17 Nov 2000 14:53:46 +0000 (GMT) X-Received: from sumac.le.ac.uk ([143.210.35.10] helo=SUMAC.cfs.le.ac.uk) by artemis.le.ac.uk with esmtp (Exim 3.16 #1) id 13wmte-00052g-00 for dros@net.bio.net; Fri, 17 Nov 2000 14:53:46 +0000 X-Received: by SUMAC.cfs.le.ac.uk with Internet Mail Service (5.5.2650.21) id <4KNP7LKZ>; Fri, 17 Nov 2000 14:52:51 -0000 X-To: "'dros@net.bio.net'" X-Mailer: Internet Mail Service (5.5.2650.21) Date: Fri, 17 Nov 2000 19:03:12 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Dear All, I've a lot of RNA in situs planned and thought about synthesising primers with either the T3 or T7 pormotor tacked onto them. Can anyone tell me the minimum amount of promotor sequence I need to use? And do I need a spacer? Thanks. Colin. Colin Moran Lab 136, Adrian Building Genetics Department Leicester University University Road LE1 7RH Tel: +44 (0)116 252 3421 cnm5@le.ac.uk --- .