From owner-dros@hgmp.mrc.ac.uk Sun Jan 21 20:58:20 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3CAD817AB2; Sun, 21 Jan 2001 20:58:19 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 0EE9117A9A; Sun, 21 Jan 2001 20:58:17 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id EFAEA17B62; Sat, 20 Jan 2001 22:59:01 +0000 (GMT) To: bionet-drosophila@net.bio.net From: hampton@hawaii.edu (Hampton Carson) Newsgroups: bionet.drosophila Subject: Undelivered Mail Returned to Sender Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 3583717AAC; Sat, 20 Jan 2001 22:58:59 +0000 (GMT) X-Received: from hawaii.rr.com (hnlmail1.hawaii.rr.com [24.25.227.33]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id D900641644 for ; Sat, 20 Jan 2001 22:58:53 +0000 (GMT) X-Received: from [24.25.228.151] ([24.25.228.151]) by hawaii.rr.com with Microsoft SMTPSVC(5.5.1877.517.51); Sat, 20 Jan 2001 12:58:51 -1000 X-Sender: hampton@pop-server.hawaii.edu X-To: dros@net.bio.net Date: Sun, 21 Jan 2001 20:58:17 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk >From: Hampton Carson >Subject: Re: MOLD PROBLEM >Cc: > >Dear Gregory: > >A simple solution is to collect a good representation from each mouldy >culture (10-20 pairs of healthy young flies) Put together in on ordinary >sterile glass vial with small medium. Change repeatedly to a new vial every >hour for a day and laater for three hours (and overnight) for about two >days and then introduce the adults into a fresh, sterile culture bottle and >discard the rest of the old culture bottles. > >This uses the principle of "serial dilution" to get rid of the >contamination. It also works well to rid cultures of mites. It uses up a >lot of vials that have to be washed and sterilized, but the flies will >efficiently clean themselves of spores fairly rapidly, dropping spores >continually in the vials. You can experiment with the number of transfers >necessary, and possibly reduce the number of transfers that I have >suggested. > >Good luck! > >Hampton Carson > > > >> I am having a horrible problem with mold contamination of my >>cultures! What would be the best method of getting rid of this problem, >>save dumping all my cultures and starting from scratch...which I really >>don't want to do!!! Any reply would be appreciated! Thanks! >> >>Gregory M. Spencer >> >>University at Albany >> >>Neurobiology Research Center >> >>Department of Biological Sciences >> >>1400 Washington Avenue >> >>Albany, New York 12222 >> >> >> >>E-mail: gs5849@albany.edu >> >>Phone: (518) 442-4335 >> >>Fax: (518) 442-4767 > > >Hampton L. Carson, Professor Emeritus >Department of Cell and Molecular Biology >University of Hawaii. >Home Address: 1314 Kalakaua Ave. Apt. 1111 >Honolulu, HI 96826 > Hampton L. Carson, Professor Emeritus Department of Cell and Molecular Biology University of Hawaii. Home Address: 1314 Kalakaua Ave. Apt. 1111 Honolulu, HI 96826 --- .