From owner-drosophila@net.bio.net Mon Sep 18 23:00:00 1995
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!solaris.cc.vt.edu!swiss.ans.net!prodigy.com!usenet
From: JHCW54B@prodigy.com (Jeanine Regenthal)
Newsgroups: bionet.drosophila
Subject: Drosophila cell culture
Date: 19 Sep 1995 19:23:49 GMT
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Hi!

     I cannot tell you how excited I was to have found this newsgroup. I 
am new to the study of Drosophila (My experience has been strictly 
mammalian cell culture). This is my second day in a project regarding 
Drosophila. I am currently interested in culturing these cells in a serum-
free environment. I have some information with respect to the reqired 
constituents of media, such as Schneider's Media. I have read a little 
about the desired concentrations of important constituents, such as: 
transferrin, albumin, b-mercaptoethanol, insulin, ethanolamine, selenium 
etc... 

     I guess I would like to know if anyone has any words of advice for 
me, i.e. personal experiences, journal articles, references or names of 
authors that I should concentrate on. 

     I have just returned to work, part-time, after having severely 
injured my back. I think it will be about a month before I can put in 
some hard time at the university library... any advice you could offer, 
so I can concentrate my efforts is immensely appreciated!

     Thankyou for your time and effort!

     Jeanine





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