From owner-cellbiol@net.bio.net Thu Dec 05 22:00:00 1996
Path: biosci![157.92.77.1]!dlombard
From: dlombard@[157.92.77.1]
Newsgroups: bionet.cellbiol
Subject: Re: transformation or transfection?
Date: 6 Dec 1996 06:24:11 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 27
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <m0vW12L-00094bC@condor.fvet.uba.ar>
NNTP-Posting-Host: net.bio.net

> To:            cellbiol@net.bio.net
> From:          BehariRN@perkin-elmer.com ("BehariRN")
> Subject:       Re: transformation or transfection?
> Date:          5 Dec 1996 11:55:11 -0800

> 
> 
> 
>      
> What is the difference between transfection and transformation of host cells by 
> recombinant vector DNA containing a chimeric gene? Is the term transfection used
> for onlywhen the recombinant DNA is introduced into eukaryotic host cells and 
> transformation for prokaryotic host cells
> 
> Thanks 
> 
> 
> 
> I don't in accord with the transformatios term will be introduced 
for prokaryotic cells. I think that transfection is the introduction 
of foraneous DNA into cells (eukariotic or prokariotic) and 
transformations is your consecueses.


Daniel M. Lombardo
Histologia y Embriologia
Facultad de Cs. Veterinarias. UBA

.