From owner-biophysics@net.bio.net Tue Jun 23 23:00:00 1998 Path: biosci!rutgers!rockyd.rockefeller.edu!news-nysernet-5.sprintlink.net!news-dc-2.sprintlink.net!news-east.sprintlink.net!news-peer.sprintlink.net!news.sprintlink.net!howland.erols.net!news.starnet.net!tulane.edu!not-for-mail From: Lee Lovejoy Newsgroups: bionet.biophysics,bionet.neuroscience Subject: Narishige electrode puller Date: Wed, 24 Jun 1998 15:00:09 -0500 Organization: Tulane University School of Medicine Lines: 10 Message-ID: <35915AC9.3A3C@mailhost.tcs.tulane.edu> Reply-To: llovejo@mailhost.tcs.tualne.edu NNTP-Posting-Host: 129.81.98.20 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I) Xref: biosci bionet.biophysics:4261 bionet.neuroscience:23650 I am using a vertical Narishige microelectrode puller. I have encountered difficulty in determining the appropriate position for the microswitches. My pippettes have two tapers; one from the gravity pull and a long, thin taper from the magnet pull. I have tried increasing the magnet, but this results in longer thin taper rather than a larger opening. Can anyone help? LL .