From owner-dros@hgmp.mrc.ac.uk Mon May 21 14:41:52 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 82ED517B20 for ; Mon, 21 May 2001 14:41:49 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id AEDBE17B1A for ; Mon, 21 May 2001 14:41:42 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 3B39517B0C; Mon, 21 May 2001 14:41:25 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E5EE617B17 for ; Mon, 21 May 2001 13:38:14 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 1546117B0F; Mon, 21 May 2001 13:38:10 +0100 (BST) To: bionet-drosophila@net.bio.net From: jaramill@mail.med.upenn.edu (Angie Jaramillo) Newsgroups: bionet.drosophila Subject: Homologous Recombination- New Method Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <200105211237.f4LCbtP10986@dolphin.upenn.edu> MIME-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 7F4E517B14 for ; Mon, 21 May 2001 13:38:08 +0100 (BST) X-Received: from dolphin.upenn.edu (DOLPHIN.UPENN.EDU [128.91.2.35]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id CB9EF17B17 for ; Mon, 21 May 2001 13:37:56 +0100 (BST) X-Received: (from jaramill@localhost) by dolphin.upenn.edu (8.11.1/8.10.1) id f4LCbtP10986 for dros@net.bio.net; Mon, 21 May 2001 08:37:55 -0400 (EDT) X-To: dros@net.bio.net X-Mailer: ELM [version 2.4 PL23-upenn3.3] Date: Mon, 21 May 2001 14:41:25 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi, I am a grad student who is increasingly frustrated using P-element mutagenesis to try to generate a null fly. I was wondering if anyone has tried Golic's Homolgous Recombination method? Or does anyone know whether his stocks are available? Any help is much apprecaited! Angie Jaramill@mail.med.upenn.edu --- .