From owner-bionews@hgmp.mrc.ac.uk Sat Feb 26 04:37:25 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B4D8A17B6F; Sat, 26 Feb 2000 04:37:23 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id A7D1617A43; Sat, 26 Feb 2000 04:37:21 +0000 (GMT) To: bionews@net.bio.net Newsgroups: bionet.announce Date: 24 Feb 2000 04:47:09 -0000 From: meton@rci.rutgers.edu (Daniel Gonzalez) Organization: Rutgers University Subject: Green Fluorescent Protein Purification Short Course Message-Id: <20000226043721.A7D1617A43@mercury.hgmp.mrc.ac.uk> Sender: owner-bionews@hgmp.mrc.ac.uk Precedence: bulk I have been asked to announce the following, The State University of New Jersey Rutgers Campus at New Brunswick Center for Research and Education in Bioluminescence and Biotechnology Programs in Biotechnology, Presents: Protein Purification: Isolation, Analysis, and Characterization of GFP, A Five and One-Half Day Hands-On Laboratory Course Using the remarkable Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression, as the source material March 12-17, 2000 May 7-12, 2000 June 4-9, 2000 July 25-28, 2000 and July 30-August 4, 2000 More than 600 scientists from around the world have strongly recommended this intensive course as an opportunity to develop protein research and analytical skills in a retreat setting. Participants work hard, identify and solve problems in the lab and enjoy camaraderie and good food and beer with colleagues. This five and one-half day laboratory course covers a wide variety of conventional methods for protein isolation, purification, and characterization. The course format integrates hands-on laboratory exercises with classroom lectures, demonstrations, study breaks, and short take-home assignments. A special feature of the course is that all laboratory work will be performed on the same starting sample (Aequorea GFP*), which will be purified from an exceedingly crude form to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing, and capillary zone electrophoresis. This feature provides a continuity of purpose, integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match. A problem-solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data, and interpret results. A student-teacher ratio not greater than 8:1 will be maintained and the faculty coordinators will be present throughout the course. *Note: The use of GFP from a recombinant source (E. coli) is also being used as starting material due to its popularity within the scientific community. For further details you can reach us, by E-mail at: meton@rci.rutgers.edu by phone at: (732) 932-9071 extension 225 by FAX at: (732) 932-3633 for a brochure and further information please visit the GFP purification short course official Web site at: http://www.rci.rutgers.edu/~meton/protein.html .