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To: bionet-drosophila@net.bio.net
From: "Olivier BLARD" <ol_blard@club-internet.fr>
Newsgroups: bionet.drosophila
Subject: Re: S2 transfections
Organization: Club-Internet (France)
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References: <a05010402b7ce4e4c1f33@[159.92.52.25]>
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Date: Thu, 20 Sep 2001 14:16:46 +0100 (BST)
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Hello,
In our lab, calcium phosphate works better than lipofectine and fugene6
on S2 cells, but the ratio of transfected cells (using GFP) is not very
good (maybe 1/30). I think DOTAP is good but we have not received this
product yet.

--

Olivier.

Louisa Flintoft <louisa.flintoft@kcl.ac.uk> a écrit dans le message :
a05010402b7ce4e4c1f33@[159.92.52.25]...
> Can anyone help me with my S2 problems? I've been transfecting using
> calcium phosphate, and I've had very mixed results in terms of
> efficiency of transfection, cell death etc. Has anyone used this
> method and got it to work consistently well? Alternatively, can
> anyone recommend an alternative method that works well?
>
> Thanks,
>
> Louisa FLintoft
>
> --
> Dr Louisa Flintoft
>
> Department of Molecular Neurobiology
> 4th Floor
> New Hunt's House
> Guy's Campus
> King's College
> London Bridge
> London SE1 9RT
>
> Lab: 020 7848 6554
> Office: 020 7848 6549
>
> ---
>


.