From owner-drosophila@net.bio.net Wed Aug 14 23:00:00 1996 Path: biosci!rutgers!uwm.edu!spool.mu.edu!news.sgi.com!swrinde!howland.erols.net!newsxfer2.itd.umich.edu!portc01.blue.aol.com!newsstand.cit.cornell.edu!cu-dialup-0052.cit.cornell.edu!user From: yol1@cornell.edu (Oliver Lung) Newsgroups: bionet.drosophila Subject: Can you identify this structure in adult female thorax Date: Wed, 14 Aug 1996 22:34:27 -0500 Organization: Cornell University Lines: 21 Sender: yol1@cornell.edu (Verified) Message-ID: NNTP-Posting-Host: cu-dialup-0052.cit.cornell.edu Hi, I'm doing immunocytochemistry on sections of adult female flies looking for targets of a secreted protein. My rudimentary knowledge of fly histology does not allow me to identify the structure that is giving a signal. On transverse sections of adult female thorax, I came across two small symetrical signals on a tubular structure in the ventral side of the thorax. This tubular structure seems to run longitudinally along the length of the body near the ventral side, but the size of the tube and the distribution of cell nuclei stained by DAPI is different in different sections (sometimes dividing the tube into two or three chambers). The image is at (http://www.tc.cornell.edu/~tsai/tmp/oliver.jpeg). The left panel is DAPI staining low mag of thorax transverse section, ventral is on top. Arrow points to two holes with no DAPI staining that is being labelled by my antibody. The right panel is a higher mag of the tubular structure, blue is DAPI staining and red is my antibody staining (arrows). The other red spots are autofluorescence. People have been telling me it could be enteric neurons, but we don't have many fly people here and no one is sure. Any help is greatly appreciated. Oliver .