From owner-celegans@net.bio.net Sun Sep 18 23:00:00 1994
Newsgroups: bionet.celegans,bionet.molbio.rapd
Path: biosci!bcm!cs.utexas.edu!news.uta.edu!wbar.uta.edu!patrick
From: patrick@wbar.uta.edu (Patrick Phillips)
Subject: RAPD on C. elegans
Message-ID: <1994Sep19.180655.9508@news.uta.edu>
Sender: news@news.uta.edu (USENET News System)
Nntp-Posting-Host: wbar.uta.edu
Organization: /usr/local/lib/news/organization
Date: Mon, 19 Sep 1994 18:06:55 GMT
Lines: 19
Xref: biosci bionet.celegans:111 bionet.molbio.rapd:775

We are just starting a project using RAPDs with everyone's favorite
nematode, C. elegans.  Is anyone out there working with this method
in C. elegans?  I would like to know which primers should for sure work
with N2 before I do a lot of playing around.

For those experienced RAPDers in b.m.rapd, what seems to be the probability
that a particular primer will amplify at all?  I've only tried four
primers so far, and none have amplified (I know the DNA and PCR reagents
are good).  I have no way of knowing whether something is going wrong with
the PCR (like the annealing temp), or if the primers just aren't hitting
anything.  Before I try 100 more primers, I'd like to know what others have
experienced.

Patrick Phillips
Biology Department
University of Texas at Arlington
patrick@wbar.uta.edu
(817) 273-2409


.