From owner-drosophila@net.bio.net Mon Feb 19 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.neca.com!news From: Mike Tormey Newsgroups: bionet.drosophila Subject: Cross-breeding Date: 20 Feb 1996 14:20:08 GMT Organization: NECAnet Lines: 14 Message-ID: <4gclap$m5k@taurus.neca.com> NNTP-Posting-Host: ip-ts1-145.neca.com I am only a student in high school, so this is a very basic question. My class is cross breeding different types of drosophila. I am breeding a group of wild type with a group of eyless type. My problem is differentiating between male and female. I was told that their gender depends on the shape of their end segment. Can anyone help me sort them out? Also, I have to knock them out so I can seperate them. My only options are to put them in my freezer, or use a device that will gas them with carbon dioxide. Neither method lasts long enough to seperate them. I have to have the first batch ready by the 22nd of this month. Can anyone help me with this problem? Thanks in advance for any help. -Mike Tormey .