From owner-drosophila@net.bio.net Wed Dec 20 22:00:00 1995
Path: biosci!agate!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!howland.reston.ans.net!torn!newshost.uwo.ca!marvin.biochem.uwo.ca!user
From: ggloor@julian.uwo.ca (Greg Gloor)
Newsgroups: bionet.drosophila
Subject: Re: Embryo injections
Date: 21 Dec 1995 14:31:32 GMT
Organization: University of Western Ontario
Lines: 19
Distribution: bionet
Message-ID: <ggloor-2112950931560001@marvin.biochem.uwo.ca>
References: <4b983u$4pa@mserv1.dl.ac.uk>
NNTP-Posting-Host: marvin.biochem.uwo.ca

In article <4b983u$4pa@mserv1.dl.ac.uk>, <Per.Kylsten@mibi.su.se> wrote:

> Hello,
> I am interested to know if anyone out there is using delta 2-3 embryos as
> recipients for transgenic constructs, on a routine basis?
> If so, are there any important alterations to standard injection protocols
> to consider (other than the obvious omission of helper plasmid) ?
> 
> Thank you.
> 
> Per.Kylsten@mibi.su.se

We use genomic delta 2-3 all the time. Just make sure that you have a way
of eliminating the third chromosome and go for it. No other alterations
needed

-- 
Greg Gloor
Dep't Of Biochemistry, UWO

.