From owner-drosophila@net.bio.net Mon May 16 23:00:00 1994 Path: biosci!bcm!NewsWatcher!user From: jb031504@mbcr.bcm.tmc.edu (Jim Bone) Newsgroups: bionet.drosophila Subject: Re: DNA prep for P1 clones? Qiagen or PEG? Followup-To: bionet.drosophila Date: Tue, 17 May 1994 16:18:21 -0600 Organization: Baylor College of Medicine Lines: 23 Message-ID: References: <2ra7um$7rn@lyra.csx.cam.ac.uk> NNTP-Posting-Host: 128.249.46.106 In article <2ra7um$7rn@lyra.csx.cam.ac.uk>, cw117@mole.bio.cam.ac.uk (Charlie Wright (Genetics)) wrote: > I'm about to do 250ml (mini in the case of P1) preps of some of the P1 > clones found on flybase. The only Protocol given says Grow O/N, > innoculate large flask, add IPTG to induce amplification, grow some more. > "proceed with the Qiagen maxi-prep according to the kit protocol". The > problem is, there isn't a Qiagen for P1 bacteriophage kit. I could treat > it like a really big plasmid, but does anyone know how the elution > buffers should be modified (if at all?) Help, help. > > Others have suggested PEG precipitation. > > Any experience appreciated. I just did a P1 DNA prep last week, and used a Qiagen Maxi (30 ml capacity) column. I grew four clones, 2 did real well (>50 ug DNA), 2 did poorly. I followed the Qiagen protocol and it turned out fine. I believe the problems are endemic to the clones I have--they just didn't grow that well. Good luck, JIM .