From owner-celegans@net.bio.net Sun Apr 28 23:00:00 1996 Path: biosci!COLORADO.EDU!tedesco From: tedesco@COLORADO.EDU (Pat Tedesco) Newsgroups: bionet.celegans Subject: Re: cosmid rescue Date: 29 Apr 1996 14:05:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Try cosmid concentrations of 1-10 ug/ml. This has worked well in our lab. In general if you are having trouble with cosmid rescue it's best to drop the concentration of cosmid. Good luck. Pat .