From owner-biophys@hgmp.mrc.ac.uk  Tue May  9 16:01:04 2000
Return-Path: <owner-biophys@hgmp.mrc.ac.uk>
Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110)
	id 0281C17A7B; Tue,  9 May 2000 16:01:02 +0100 (BST)
Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41])
	by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id QAA09510
	for <biophys-list@hgmp.mrc.ac.uk>; Tue, 9 May 2000 16:00:58 +0100 (BST)
Received: (from news@localhost)
	by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id QAA16425
	for biophys-list@hgmp.mrc.ac.uk; Tue, 9 May 2000 16:00:56 +0100 (BST)
X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to <news> using -f
From: Oliver <oliver@biophysik.biologie.uni-mainz.de>
X-Newsgroups: bionet.biophysics
Subject: pH-measurement in protein containing samples
Date: Tue, 09 May 2000 17:02:07 +0200
Organization: Johannes Gutenberg-Universitaet Mainz, Germany
Lines: 16
Message-ID: <3918286F.F42B67D9@biophysik.biologie.uni-mainz.de>
Mime-Version: 1.0
Content-Type: text/plain; charset=iso-8859-1
Content-Transfer-Encoding: 8bit
X-Trace: bambi.zdv.Uni-Mainz.DE 957884452 7364 134.93.248.74 (9 May 2000 15:00:52 GMT)
X-Complaints-To: usenet@mail.uni-mainz.de
X-Mailer: Mozilla 4.7 [de] (Win95; I)
X-Accept-Language: de
To: biophys@hgmp.mrc.ac.uk
Sender: owner-biophys@hgmp.mrc.ac.uk
Precedence: bulk

Hello,
I try to use a common pH-electrode (Ingold) to measure pH changes upon
oxygenation of hemocyanin of spiders. The problem is, that I have to use

concentrations between 22 to 32 g/l. The protein is denaturated inside
the diaphragm of the electrode after a short time. Does anybody use the
Friscolyt-electrolyt (Mettler-Toledo) to solve that problem?
 I also donīt know how to change the electrolyte. Itīs a mico-electrode
and capillary forces prevent simple change by pipette.

Thank you for any help,

Oliver





.