From owner-celegans@net.bio.net Thu Apr 15 23:00:00 1999
Path: biosci!biosci!not-for-mail
From: mcunni2@lsumc.edu (Cunningham, Melody K)
Newsgroups: bionet.celegans
Subject: two extrachromosomal arrays
Date: 15 Apr 1999 18:53:24 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Hi!  I'm trying to isolate a gene based on how it affects gfp expression.  I

have crossed my mutant gene (integrated) into a strain that has mec-7gfp
(extrachromosomal array).  To rescue my favorite gene, I will be crossing N2

transgenics containing cosmids within the region of my favorite gene and
looking for a change in gfp expression.  This means that I will end up with
a
strain that has two extrachromosomal arrays.  How do strains that contain
more than one extrachromosomal arrays behave?  Will this pose a problem for
me?
If anyone can help, please email me at the following address:
mcunni2@lsumc.edu

Thanks,
Melody Cunningham


William R. Morgan
931 College St.
Department of Biology			Phone:  330-263-2026
The College of Wooster                  FAX:    330-263-2378
Wooster, OH  44691                      E-mail: wmorgan@acs.wooster.edu



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