From owner-drosophila@net.bio.net Mon May 16 23:00:00 1994
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!lyra.csx.cam.ac.uk!mole.bio.cam.ac.uk!cw117
From: cw117@mole.bio.cam.ac.uk (Charlie Wright (Genetics))
Newsgroups: bionet.drosophila
Subject: DNA prep for P1 clones?  Qiagen or PEG?
Date: 17 May 1994 10:57:26 GMT
Organization: University of Cambridge, England
Lines: 19
Message-ID: <2ra7um$7rn@lyra.csx.cam.ac.uk>
NNTP-Posting-Host: mole.bio.cam.ac.uk
Summary: as subject
Keywords: P1, PEG, Qiagen

I'm about to do 250ml (mini in the case of P1) preps of some of the P1 
clones found on flybase.  The only Protocol given says Grow O/N, 
innoculate large flask, add IPTG to induce amplification, grow some more. 
"proceed with the Qiagen maxi-prep according to the kit protocol".  The 
problem is, there isn't a Qiagen for P1 bacteriophage kit.  I could treat 
it like a really big plasmid, but does anyone know how the elution 
buffers should be modified (if at all?)  Help, help.

Others have suggested PEG precipitation.

Any experience appreciated.


Charlie
-- 
C. R. Wright                                    Dept. of Genetics
+44 (0)223 333970 telephone                     Univ. of Cambridge
+44 (0)223 333992 telefax                       Downing Street, Cambs.
cw117@mole.bio.cam.ac.uk                        CB2 3EH, England

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