From owner-drosophila@net.bio.net Tue Dec 19 22:00:00 1995 Path: biosci!MIBI.SU.SE!Per.Kylsten From: Per.Kylsten@MIBI.SU.SE Newsgroups: bionet.drosophila Subject: Embryo injections Date: 20 Dec 1995 06:20:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello, I am interested to know if anyone out there is using delta 2-3 embryos as recipients for transgenic constructs, on a routine basis? If so, are there any important alterations to standard injection protocols to consider (other than the obvious omission of helper plasmid) ? Thank you. Per.Kylsten@mibi.su.se .