From owner-biophysics@net.bio.net Mon Apr 13 23:00:00 1998 Path: biosci!rutgers!rockyd.rockefeller.edu!newsfeed.nyu.edu!newsfeed.gte.net!howland.erols.net!csir.uni.net.za!und.uni.net.za!und.ac.za!newsadmin From: "che" Newsgroups: bionet.biophysics,bionet.cellbiol,bionet.cellbiol.cytonet,bionet.immunology,bionet.metabolic-reg,bionet.molbio.ageing,bionet.molbio.methds-reagnts,bionet.molbio.proteins Subject: Cytosolic Buffer Date: 14 Apr 1998 11:35:19 GMT Organization: University of Natal Lines: 17 Message-ID: <01bd6799$a1398300$df80808f@bchm7.bch.unp.ac.za> NNTP-Posting-Host: bchm6.bchm.unp.ac.za X-Newsreader: Microsoft Internet News 4.70.1155 Xref: biosci bionet.biophysics:4133 bionet.cellbiol:9234 bionet.cellbiol.cytonet:1317 bionet.immunology:13824 bionet.metabolic-reg:2046 bionet.molbio.ageing:3927 bionet.molbio.methds-reagnts:66510 bionet.molbio.proteins:12647 Hello All, Are there any available buffers (commercial or recipes will do) that mimick the cytosolic environment. These buffers would presumably provide similar pH, ionic and reducing strengths as the cytosol. I am working with isolated lysosomes, and would like to provide them with a 'homely' environment without the interference of other organelles. Thank you in advance Che Pillay Dept. of Biochemistry University of Natal (PMB) Scottsville South Africa Pillayc@agric.unp.ac.za .