From owner-dros@hgmp.mrc.ac.uk Thu Mar 21 14:00:54 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B9AEE17B14 for ; Thu, 21 Mar 2002 14:00:52 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id CA71B17B03 for ; Thu, 21 Mar 2002 14:00:44 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 3114417AB3; Thu, 21 Mar 2002 14:00:42 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B61C317AF2 for ; Wed, 20 Mar 2002 22:51:29 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 5028617A97; Wed, 20 Mar 2002 22:51:28 +0000 (GMT) To: bionet-drosophila@net.bio.net From: kathleef@sfu.ca ("kathleen_fitzpatrick@popserver.sfu.ca") Newsgroups: bionet.drosophila Subject: yellow, heatshock, Gal4 vector Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3.0.6.32.20020320155701.007feb00@popserver.sfu.ca> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 265A617AF2 for ; Wed, 20 Mar 2002 22:51:26 +0000 (GMT) X-Received: from rm-rstar.sfu.ca (rm-rstar.sfu.ca [142.58.120.21]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id C0EC717A97 for ; Wed, 20 Mar 2002 22:51:22 +0000 (GMT) X-Received: from hondalab1.sfu.ca (hondalab1.mbb.sfu.ca [142.58.209.22]) by rm-rstar.sfu.ca (8.10.1/8.10.1/SFU-5.0H) with SMTP id g2KMpKn20888 for ; Wed, 20 Mar 2002 14:51:20 -0800 (PST) X-Sender: kathleef@popserver.sfu.ca X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) X-To: dros@net.bio.net Date: Thu, 21 Mar 2002 14:00:42 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi there I am interested in constructing a P element transformation vector which has the following: marked with yellow+ instead of white or rosy, a heat shock promoter driving Gal4 expression to which I might fuse a gene or genes to make Gal4 fusion proteins. I can try to sew this together myself, but it occurred to me that someone might have a vector out there with part of this already on it, like the hs promoter and the yellow+. If anyone does, would you be willing to share a small aliquot of it along with a map? If so, please email me at: kathleef@sfu.ca Thanks very much Kathleen Kathleen Fitzpatrick MBB 8888 University Dr. Simon Fraser University Burnaby BC V5A 1S6 ph:604 291-4856 fax:604 291 5583 "The earth is like a tiny grain of sand, only much, much heavier" --- .