From owner-dros@hgmp.mrc.ac.uk Wed Sep 19 22:47:23 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9968717B66 for ; Wed, 19 Sep 2001 22:47:22 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 0D0F317B9D for ; Wed, 19 Sep 2001 22:47:20 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 7852417B66; Wed, 19 Sep 2001 22:47:16 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 4B8FD17B78 for ; Wed, 19 Sep 2001 14:41:16 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id BDB9417B77; Wed, 19 Sep 2001 14:41:05 +0100 (BST) To: bionet-drosophila@net.bio.net From: louisa.flintoft@kcl.ac.uk (Louisa Flintoft) Newsgroups: bionet.drosophila Subject: S2 transfections Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" ; format="flowed" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3AA6C17A9F for ; Wed, 19 Sep 2001 14:40:59 +0100 (BST) X-Received: from angelo.kcl.ac.uk (angelo.kcl.ac.uk [137.73.66.5]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B4C5017B7A for ; Wed, 19 Sep 2001 14:40:39 +0100 (BST) X-Received: from [159.92.52.25] (pc052-025.devn.umds.ac.uk [159.92.52.25]) by angelo.kcl.ac.uk with ESMTP id f8JDedA28214 for ; Wed, 19 Sep 2001 14:40:39 +0100 (BST) X-Sender: stna1804@mail.kcl.ac.uk X-To: dros@net.bio.net Date: Wed, 19 Sep 2001 22:47:16 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Can anyone help me with my S2 problems? I've been transfecting using calcium phosphate, and I've had very mixed results in terms of efficiency of transfection, cell death etc. Has anyone used this method and got it to work consistently well? Alternatively, can anyone recommend an alternative method that works well? Thanks, Louisa FLintoft -- Dr Louisa Flintoft Department of Molecular Neurobiology 4th Floor New Hunt's House Guy's Campus King's College London Bridge London SE1 9RT Lab: 020 7848 6554 Office: 020 7848 6549 --- .