From owner-drosophila@net.bio.net Fri Mar 08 22:00:00 1996 Path: biosci!molbio.su.se!mitch From: mitch@molbio.su.se (Mitch Dushay) Newsgroups: bionet.drosophila Subject: inducible promoter Date: 9 Mar 1996 02:21:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Roger Jacobs wrote: Ectopic expression driven by heat shock activation of hsp70 or hsp82 in adult Drosophila can induce phenocopies that complicate analysis. Does anyone know of an efficient way to activate these promoters without heat? I hope he gets good answers. I'd like to ask a broader question. What other inducible promoters are available for fly work? I believe the methotrexate promoter turns on with exposure to copper. The cecropin promoter turns on in fat body and hemocytes in response to injury and infection. Ideally, one would want a not-too-leaky promoter that can be activated with minimal experimenter effort and fly stress. Is someone out there working on such an inducible gene whose promoter could be harnessed? Thanks for any and all suggestions. mitch@molbio.su.se .