From owner-drosophila@net.bio.net Thu Aug 04 23:00:00 1994 Newsgroups: bionet.drosophila,bionet.general,bionet.molbio.methds-reagnts Path: biosci!bcm!news.msfc.nasa.gov!europa.eng.gtefsd.com!howland.reston.ans.net!torn!news.cs.uwindsor.ca!sinisac From: sinisac@server.uwindsor.ca (Sinisac Mike) Subject: ** Help with AO staining of Drosophila salivary glands ** Message-ID: Sender: news@uwindsor.ca (Usenet) Organization: University of Windsor, Ontario, Canada Date: Fri, 5 Aug 1994 22:48:10 GMT Lines: 12 Xref: biosci bionet.drosophila:494 bionet.general:10598 bionet.molbio.methds-reagnts:16999 I would like to know if anyone has a staining protocol for staining the salivary glands of drosophila with Acridine Orange. I am interested in viewing the differentiation between the RNA (chromosome puffs) and DNA portions of the polytene chromosomes located in the salivary glands of another class of diptera. I've tried several things already and am have been unable to come up with anything really good yet. Any help would be much appreciated. Dave sinisac@server.uwindsor.ca .