From owner-celegans@net.bio.net Wed Aug 17 23:00:00 1994
Path: biosci!MEDSCI.MBP.MISSOURI.EDU!gruidl
From: gruidl@MEDSCI.MBP.MISSOURI.EDU (Mike Gruidl)
Newsgroups: bionet.celegans
Subject: microinjection of DNA
Date: 18 Aug 1994 11:37:32 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Dear worm netters:

I am getting ready to do microinjection rescue and I want to know if I
need to use CsCl purified DNA.  I know about problems with DNA ppt with Na
salts and that I should use K salts.  I would guess that there would be
fewer problems with CsCl DNA, but does an alklaine lysis miniprep DNA work OK?

Just a note.  As long as I use enough DNA, I am able to do most
manipulations with miniprep DNA including sequencing.  My only concern is
how to determine how much DNA I am using or is that not a problem as long
as I have enough and I don't clog the needle.

please email any advise to gruidl@medsci.mbp.missouri.edu

Thanks 

Mike Gruidl




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