From owner-dros@hgmp.mrc.ac.uk Thu Sep 19 22:24:15 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 3D06817B19 for ; Thu, 19 Sep 2002 22:24:14 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B4DAC17B80 for ; Thu, 19 Sep 2002 22:24:12 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 438F617B66; Thu, 19 Sep 2002 22:24:11 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E306117B1A for ; Thu, 19 Sep 2002 19:11:02 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 5985117A8A; Thu, 19 Sep 2002 19:11:01 +0100 (BST) To: bionet-drosophila@net.bio.net From: rabi307@uvm.haifa.ac.il (Eugenia Rashkovetsky) Newsgroups: bionet.drosophila Subject: DNA pooling Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3D8A218C.F62053CE@uvm.haifa.ac.il> MIME-Version: 1.0 Content-Type: text/plain; charset=x-user-defined Content-Transfer-Encoding: 7bit X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 1843517A5C for ; Thu, 19 Sep 2002 19:10:10 +0100 (BST) X-Received: from research.haifa.ac.il (research.haifa.ac.il [132.74.1.32]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9925C17A8A for ; Thu, 19 Sep 2002 19:10:07 +0100 (BST) X-Received: from uvm.haifa.ac.il ([132.74.22.183]) by research.haifa.ac.il (8.9.3/8.9.3) with ESMTP id VAA28760 for ; Thu, 19 Sep 2002 21:10:06 +0300 X-Mailer: Mozilla 4.76 [en] (Win98; U) X-Accept-Language: en,ru X-To: dros@net.bio.net Date: Thu, 19 Sep 2002 22:24:11 +0100 (BST) X-Razor-id: 2f29c813c90456c00b6e801a3b9657cdfe4463d9 Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk DNA pooling is a genetic screening method that combines DNA from many individuals in a single polymerase chain reaction (PCR) reaction to generate a representation of allele frequencies. It is done in human and many other animals. But I have no idea how many individual I should take for my analysis in Drosophila So I want to know that how many individuals will be used for DNA pooling in Drosophila. If somebody has idea please let me know to my e-mail address below. I will be gratefull for this kind help. Shree Ram Singh singhsr2000@yahoo.com --- .