From owner-metabolic-reg@net.bio.net Thu Jun 20 23:00:00 1996 Path: biosci!rutgers!gatech!usenet.eel.ufl.edu!news.ultranet.com!homer.alpha.net!uwm.edu!news.cse.psu.edu!news.ecn.bgu.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in2.uu.net!newsflash.concordia.ca!sunqbc.risq.net!athena.ulaval.ca!mac107.peps.ulaval.ca!user From: elauzier@fse.ulaval.ca (Edouard Lauzier) Newsgroups: bionet.molbio.methds-reagnts,bionet.info-theory,bionet.molbio.proteins,bionet.metabolic-reg,bionet.cellbiol Subject: Beginner with RNA extraction Date: 21 Jun 1996 14:57:38 GMT Organization: Graduate Student ( LABSAP U. Laval ) Lines: 21 Message-ID: NNTP-Posting-Host: mac107.peps.ulaval.ca Xref: biosci bionet.molbio.methds-reagnts:46054 bionet.info-theory:4153 bionet.molbio.proteins:8172 bionet.metabolic-reg:773 bionet.cellbiol:4966 Sorry for the silly question but... I know that the 260/280 ration tells u the purity of your sample ( 2.0 for RNA ) Lately I'm unable to get over 1.5 I understand that the 260 value tells you the amount of nucleic acid you have... But what the 280 means ? How can I loer it to get a higher ratio ? My point being if phenol ( 270 ) or proteins (230 ) absorb at those wavelenghts, the fact of getting rid of them should not affect the ratio... Thanks in advance for your help... p.s. I use the Chomczynski and Sacchi ( 1986 ) method... -- Edouard Lauzier, B.Sc. elauzier@fse.ulaval.ca Physical Activity Science Laboratory (Laval University) G1K 7P4 CANADA .