From owner-celegans@net.bio.net Sun Mar 29 23:00:00 1998 Path: biosci!biosci!not-for-mail From: jbrowne@may.ie Newsgroups: bionet.celegans Subject: RT-PCR Control Primers Date: 30 Mar 1998 06:30:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Approved: wmorgan@acs.wooster.edu Distribution: world Message-ID: <6foa9h$j7r@net.bio.net> NNTP-Posting-Host: net.bio.net I am currently using RT-PCR to examine genes in a nematode closely related to C. elegans and need a positive control for the reactions. Can anyone suggest a primer pair for RT-PCR which span an intron, are ubiquitously expressed and highly conserved. Thanking you in advance for all suggestions. John Browne jbrowne@ailm.may.ie .