From owner-diagnostics@net.bio.net Wed Mar 22 22:00:00 1995
Path: biosci!PLAINS.NODAK.EDU!dwatson
From: dwatson@PLAINS.NODAK.EDU (David Watson)
Newsgroups: bionet.diagnostics
Subject: PCR at high altitude
Date: 23 Mar 1995 14:36:08 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <199503232235.AA11783@plains.NoDak.edu>
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Dear Netters:

A quick observation (somebody correct me if this is not accurate):

Taq is an enzyme, albeit one with a very high temperature optimum.
Therefore, at a temp slightly below boiling in Quito (89 C, was it?),
shouldn't Taq still work, even if slightly less than optimally, such that
routine amplifications could still be accomplished? Does anyone know how
rapidly Taq's efficiency falls off either side of 94 C? I assume the dropoff
is steeper above 94 C?
 |===========================================|
  David A. Watson, Ph.D. (dwatson@plains.nodak.edu)  
  Asst. Prof. of Veterinary and Microbiological Sciences       
  North Dakota State University 
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                   "Some are wise, some otherwise."                           
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