From owner-celegans@net.bio.net Fri Aug 23 23:00:00 1996
Path: biosci!agate!garriga4.berkeley.edu!user
From: baum@mendel.berkeley.edu (Paul Baum)
Newsgroups: bionet.celegans
Subject: Blue Fluorescent Protein
Date: Sat, 24 Aug 1996 11:27:39 -0800
Organization: UCBerkeley
Lines: 11
Message-ID: <baum-2408961127390001@garriga4.berkeley.edu>
NNTP-Posting-Host: garriga4.berkeley.edu

We would like to use blue fluorescent protein for double labeling
alongside GFP.  Has anyone in the worm community tried using the
double-mutant BFP protein Y66H Y145F described by Heim and Tsien in
Current Biology 1996 vol. 6 no. 2?  Can the fluorescence be distinguished
from GFP by using normal or special filter sets?  Is the bleaching so bad
that the BFP signal cannot be detected with high speed film or a CCD
camera?

Thanks

Paul

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