From owner-celegans@net.bio.net Wed May 21 23:00:00 1997
Newsgroups: bionet.celegans
Path: biosci!webtv.net!su-news-feed4.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.direct.ca!torn!utnut!utzoo!guy
From: guy@zoo.utoronto.ca ( Guy Tanentzapf)
Subject: Gfp antibodies
X-Newsreader: TIN [version 1.2 PL2]
Organization: U of Toronto Zoology
Message-ID: <EAK8xt.95M.F@zoo.utoronto.ca>
Date: Thu, 22 May 1997 02:15:27 GMT
Lines: 12

Dear Worm Breeders,

I have made a fusion of gfp and MFG (my favorite gene) and I do see some
expression. However this expression is somewhat weak. I thought of 
using anti-GFP antibodies to stain and thus amplify my signal. And was 
wondering if anyone has tried this before (stained gfp-fusion worms 
with anti-gfp antibodies)?. If so, could you recommend a commercial brand 
of anti-GFP antibody that you found works best?

thanx,

Guy

.