From owner-metabolic-reg@net.bio.net Thu Sep 26 23:00:00 1996
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From: "Achim Recktenwald, PhD" <achim@ibex.ca>
Newsgroups: bionet.metabolic-reg
Subject: Re: Very high MW determination?
Date: Fri, 27 Sep 1996 08:24:45 -0500
Organization: IBEX Technologies, Inc., Biochemistry, 5485 Pare, Montreal, PQ, H4P 1P7, Canada
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To: "Nigel J.Osborn" <nigel@njosborn.demon.co.uk>

Nigel J.Osborn wrote:
> 
> Does anyone know how to measure protein MW in the order of 500,000 to
> several million? Any help would be appreciated.
> 
> Nigel.


Depends how exact you need it. 
You could try gel-filtration with gels having pore sizes large enough
for viruses.
I would try Sepharose 2B or CL-2B.

Achim

.