From owner-diagnostics@net.bio.net Sun Dec 29 22:00:00 1996
Path: biosci!biosci!not-for-mail
From: tjiffar@marlin.utmb.edu
Newsgroups: bionet.diagnostics
Subject: Transformation of human T-cells with Herpesvirus saimiri
Date: 30 Dec 1996 11:42:34 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Approved: kevin@embra.compulink.co.uk
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I am interested in transforming CD8+ T cell clones with Herpesvirus 
saimiri, and I was wondering if anyone who has had success (or failure) with 
this could give me advice about aspects of the project which might not be 
found in the current literature. I am trying to find out about aspects of 
protocols which are not explicitly detailed in manuscripts, but which might 
be critical for success. Also, how difficult is it to successfully carry out 
this procedure?



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