From owner-drosophila@net.bio.net Tue Nov 07 22:00:00 1995
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!newsfeed.pitt.edu!godot.cc.duq.edu!news.duke.edu!ocarina.cellbio.duke.edu!user
From: Marc_Champagne@cellbio.duke.edu (Marc Champagne)
Newsgroups: bionet.drosophila
Subject: Re: Tell me how to prepare the 25kb plasmid DNA
Date: 8 Nov 1995 00:14:21 GMT
Organization: Duke Cell Biology
Lines: 21
Message-ID: <Marc_Champagne-0711951919370001@ocarina.cellbio.duke.edu>
References: <47eluc$5o0@sun330.snu.ac.kr>
NNTP-Posting-Host: ocarina.cellbio.duke.edu

In article <47eluc$5o0@sun330.snu.ac.kr>, () wrote:

>    Hello, 
>    My name is Dongkook Park, graduate student of Seoul National
> Student and I'm studying the molecular genetics of Drosphila
> melanogaster.
>    BTW, I have the obstacle to prepare the transposon plasmid for
> transformation.
>   I have subcloned 15kb DNA fragment at the pCaSpeR vector.
>   So, I confirmed the clone through  mini prep.
>  But, I does not  prepare the more amount of it by medium prep.
> 
>   Please, mail me how to prepare the large size DNA prep 
>   whoever knows the tip.
>   
>   My address is followed;
>      dkpark@power4.snu.ac.kr

Did you use the Promega wizard mini-preps?  If so, I know that their silica
matrix sticks so much to the DNA that >20kb DNA won't get eluted.  You might
want to check for a homemade prep (see Maniatis).

.