From owner-diagnostics@net.bio.net Wed Mar 22 22:00:00 1995
Path: biosci!NCCCOT.AGR.CA!WHEATCROFTR
From: WHEATCROFTR@NCCCOT.AGR.CA
Newsgroups: bionet.diagnostics
Subject: Re: low boiling point
Date: 22 Mar 1995 20:10:37 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 21
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <01HOGBWKERZ6001C7V@GW.AGR.CA>
NNTP-Posting-Host: net.bio.net

 
>On 21 Mar 1995 WHEATCROFTR@ncccot.agr.ca wrote:

> Elevate the boiling point by adding DMSO

>Pardon me, but a think that using DMSO is not a good idea, since
>=BFf=3D=A7=ACN=E9{=ABE=B4=E4x=AANx<=B7=FE=D2'=D9"=B7=CC=A6LMW}2y=E3=F0=EB=
>=B4=EF=BE=A0 p4D=C5&=D6 =E6=B6=ACc7=A7'D&$=EADMSO brakes
>hidrogen bonds and it interferes with the anealing process.DMSO is used in
>strand separation procedures.=20
>=A30=09=AA}"=C1=AF=E4V=D8=DB<W=B9f=DB=F7t=CA=AEq=DDL1=A48=FC=CE=C82=E6

>Return-path: <dsleal@academ01.mty.itesm.mx>
 
Dear Diana and other DMSO doubters,

See Shen et al, Trends in Genetics 8:227 (1992). Title: DMSO improves PCR...
I think they had 5% in their samples. This should push the BP up enough for 
Quito. [I believe the BP of neat DMSO is 189 C at sea level]

Roger W.

.