From owner-drosophila@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!LAMAR.COLOSTATE.EDU!ashok From: ashok@LAMAR.COLOSTATE.EDU (Ashok Mudgapalli) Newsgroups: bionet.drosophila Subject: Amplification of large fragments of DNA! Date: 9 Jan 1996 20:54:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, Does any body has good experience in amplifying 15 kb or larger size of Drosophila genomic fragment? I am sure some of you might have done PCR amplification of such fragments. I appreciate if some body could provide me the information about best PCR kit available in the market. As every body must be aware of the fact that one need to have highest quality of genomic DNA for large fragment amplification, I would also appreciate if some body could provide me the method or reference to prepare the highest quality genomic DNA for this particular application. I was all the time preparing the genomic DNA from flies using classical method ( Phenol:Chloroform extractions) which was working pretty well for most of the molecular biology application. At this point I am not sure whether I can use same DNA for PCR amplification of large fragments. Some recent refernces are highly appreciated Thanks in advance to every body Ashok Mudgapalli .