From owner-drosophila@net.bio.net Thu Mar 07 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.cais.net!chi-news.cic.net!newsxfer2.itd.umich.edu!news.itd.umich.edu!usenet From: Krista Golden Newsgroups: bionet.drosophila Subject: X-gal staining live larvae Date: Fri, 08 Mar 1996 14:24:01 -0800 Organization: University of Michigan Lines: 10 Message-ID: <3140B381.3EC@umich.edu> NNTP-Posting-Host: bodmerpc1.biology.lsa.umich.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Does anyone know of a method for using X-gal to stain enhancer trap lines as live larvae. I've heard that the method involves the larvae eating(?) the X-gal somehow. However, I can't seem to find any references for that. If you know how to do it or know of a reference please let me know. Thank you in advance, Krista Golden kgolden@umich.edu .