From owner-celegans@net.bio.net Mon Jan 22 22:00:00 1996 Path: biosci!MAIL.MISSOURI.EDU!c621617 From: c621617@MAIL.MISSOURI.EDU (SMITHPA) Newsgroups: bionet.celegans Subject: Questions on making worm antibodies in chickens, mice, rabbits Date: 23 Jan 1996 08:09:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601231602.KAA44910@mail.missouri.edu> NNTP-Posting-Host: net.bio.net We have synthesised two C. elegans peptides and are trying to pick animal species to immunize to produce antibody. We were wondering what experiences other labs have had with chicken, rabbit, and mice as far as pre-exposure to worm proteins as seen in pre-immune western blot and as far as reactivity to worm protein after immunization. Thank you, Pliny A. Smith .