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Subject: BioTechniques 22(5), May 1997
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BioTechniques 22(5), May 1997
Table of Contents

BENCHMARKS

In vivo labeling of over-expressed
recombinant proteins in E. coli
     Giovane, C., Schwalbach, G., and
     Weiss,E.

Expression of the highly toxic centromere
binding protein CENP-B in E. coli using the
pET system in the absence of the inducer
IPTG.=20
     Bocanegra, J.A., Bejarano, L.A., and
     Valdivia, M.M.

Increased efficiency in screening large
numbers of cDNA fragments generated by
differential display
     Corton, J.C. and Gustafsson, J.-A.

XcmI-containing vector for direct cloning of
PCR products
     Borovkov, A.Y. and Rivkin, M.I.

High efficiency human B-cell cloning
using hygromycin B-resistant feeder cells
     Fabb, S.A. and Ragoussis, J.

Rapid mapping and subcloning of genomic
clones in bacteriophage lambda by PCR
          Woo, H.-H. and Hawes, M.C.

PCR-based assay to determine nuclear matrix
association
     Kramer, J.A. and Krawetz, S.A.

Spatial visualization of apoptosis using a
whole-mount in situ DNA end-labeling
technique.
     Smith, S.M. and Cartwright, M.M.

Direct in situ end labeling for detection of
apoptotic cells in tissue sections
     Zhang, Z. and Galileo, D.S.

Optimized factor V gene mutation detection
using buffy-coat direct PCR
     Pernod, G., Mossuz, P., and Polack, B.


Spectrophotometric determination of oxidative
metabolism.=20
     Lokuta, M.A., Mehring, G.H., and
     Paulnock, D.M.

Protein staining with Ponceau S during
acid-urea-triton-polyacrylamide gel
electrophoresis
     Correia, H., Rivero, J., and Herrera, F.

Isolation of nuclear proteins from human
brains
     Zhang, X., Huang, C.J., Nazarian, R.,
     Ritchie, T., de Vellis, J.S., and Noble,
     E.P.

Use of a single sequencing termination
reaction to distinguish between cytosine and
5-methylcytosine in bisulfite-modified DNA
     Yuanxiang, Z., Magill, J.M., Newton,
     R.J., and Magill, C.

Hyaluronidase generates a single-cell
suspension from cultured mouse lung
epithelial cells.
     Tolwinski, N.S. and Kano-Sueoka, T.

pPE1000: a versatile vector for the
expression of epitope-tagged foreign proteins
in transgenic plants
     Hancock, K.R., Phillips, L.D., White,
     D.W.R., and Ealing, P.M.

Two products for siliconizing glass
plates used in gel electrophoresis
     Elsom, B.L. and Herzog, N.K.

Rapid method for preparing adenovirus DNA
     Deryckere, F. and Burgert, H.-G.

Microplate digestion of minipreps from
a single microcentrifuge tube
     Brady, J.L. and Lew, A.M.

Isolation of genomic DNA from claw clippings
for genetic analysis by PCR
     Drummond, P.B., Song, Y., Vaughn, D.,
     Gbadamosi, A., Butler, K., and Smith,
     E.J.

Increased yield of plasmid DNA during removal
of CsCl by ethanol precipitation
     Hildeman, D.A. and Muller, D.

Determining the potentiative state of a
chromatin domain
     Kramer, J.A. and S.A. Krawetz


THE INTERNET ON-RAMP
     -Cyberspace for Biologists


SHORT TECHNICAL REPORTS

PCR-amplified cDNA probes for verification of
differentially expressed genes
     Ross, R., Kumpf, K., and Reske-Kunz,
     A.B.

Agglutination-inhibition assay for the
detection of recombinant proteins tagged with
peptideepitopes
     Dolezal, O., Coia, G., and Hudson, P.J.

Transient selection during vaccinia virus
recombination with insertion vectors without
selectable markers.=20
     Kurilla, M.G.

Cloning DNA fragments between two
adjacent/overlapping restriction sites using
a "positive stuffer"
     Loukianov, E., Loukianova, T., and
     Periasamy, M.

Making hybrids of two-hybrid systems
     Dagher, M.-C. and Filhol-Cochet, O.

Adaptation of a =E1-1,3-glucanase assay
to microplate format
     Zheng, Y. and Wozniak, C.A.

Association of enzyme inhibition with methods
of museum skin preparation
     Hall, L.M., Willcox, M.S., and Jones,
     D.S.

Detection of mycoplasma infection of
mammalian cells
     Xia, H., Fitzgerald, J., Bredt, D.S.,
     and Forsayeth, J.R.


RESEARCH REPORTS

An iterative and regenerative method for DNA
sequencing
     Jones, D.H.

Alternative system for detection and mapping
of activation domains
     Askovic, S. and Baumann, R.

Optimization of cellular ELISA for assay of
surface antigens on human synoviocytes
     Smith, D.D., Cohick, C.B., and Lindsley,
     H.B.

Microplate assay for measurement of histamine
release from mast cells
     Price, J.A.

Simultaneous detection of multiple point
mutations using fluorescence-coupled
competitive primer extension
     Fauser, S. and Wissinger, B.

Collagen-coated barium-alginate microcarriers
for the culture of anchorage-dependent
mammalian cells
     Grohn, P., Klock, G., and Zimmermann, U.


PRODUCT APPLICATION FOCUS

Agarose-based system for separation of short
tandem repeat loci
     White, H.W. and N. Kusukawa

The PerFect Lipid Optimizer Kit for
maximizing lipid-mediated transfection of
eukaryotic cells
     Griffiths, T., Russell, M., Froning, K.,
     Brown, B.D., Scanlon,S.M., Almazan, M.,
     Marcil, R., and Hoeffler, J.P.


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