From owner-celegans@net.bio.net Wed Nov 16 22:00:00 1994 Path: biosci!FRODO.MGH.HARVARD.EDU!BURGLIN From: BURGLIN@FRODO.MGH.HARVARD.EDU (Thomas Burglin) Newsgroups: bionet.celegans Subject: dominant markers Date: 16 Nov 1994 16:43:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 157 Sender: daemon@net.bio.net Distribution: world Message-ID: <941116194333.2040645d@Frodo.MGH.Harvard.EDU> NNTP-Posting-Host: net.bio.net From: SMTP%"patterson@frodo.mgh.harvard.edu" 16-NOV-1994 19:10:54.90 To: BURGLIN CC: Subj: dominant markers To: celegans@net.bio.net From: patterson@frodo.mgh.harvard.edu (Garth I. Patterson) Subject: dominant markers Date: 16 Nov 1994 16:59:46 GMT Message-ID: NNTP-Posting-Host: atgw-wel-900d-1-c14.mgh.harvard.edu As I promised, I have collated the answers I got to the following post from last week: >I would like to be able to do crosses with some existing strains used as >females. These strains have no markers that will allow me to identify >hermaphrodite cross progeny. Are there any dominant markers that I >could introduce into my male strain that I can use to let me identify cross >progeny? One suggestion that I have already gotten is a lin-12 dominant >allele. However, the hermaphrodites that result will be unable to mate, >which is not the most convenient for me. If anyone knows of a dominant >marker that allows males to mate, e-mail it to me. I will post a summary >of the results. As you can see, I got a terrific response. You have your choice of a large number of dominant markers. I have done minimal editing of people's responses to get this summary into an easily read format. Thanks to everyone for your help! ced-9(n1950) ces-1(n703) --For both you would have to find cross-progeny under Nomarski, which is a pain in the butt. from hengartn@cshl.org (Michael Hengartner) deg-1 (u38). --I use deg-1(u38), which is X-linked, so the males are hemizygous. It is tricky to score, but it has worked for me. (from rhind@mendel.Berkeley.EDU ( Nick Rhind)) dpy-5 (e61)I dpy-13 (e184)IV Several dpys also work, but you need a practiced eye: Strategy: het males mate, score semi-dpy progeny.From: cori@itsa.ucsf.EDU (Cori Bargmann) dpy-13(e184)IV --I always had really good luck with dpy-13(e184). It's a semi-dominant, so that dpy-13/+ is a semi-dpy male which mates quite well. You'll only pick up half your cross progeny, though. (from jamison@csl.ncsa.uiuc.edu (Curtis Jamison) and from David.Greenstein@mcmail.vanderbilt.edu) dpy-10 dpy-11 Some people can score dpy-10/+ and dpy-11/+, but they are almost subliminal.From: cori@itsa.ucsf.EDU (Cori Bargmann) dpy-25(e817sd) II --dpy-25(e817sd) II/+ hets are semi-dpy, nonetheless males are rather good maters. (from lmichel@lgme.u-strasbg.fr Michel Labouesse) flu-3 (from JJTHADEN@life.uams.edu) --flu-3, which according to Siddiqui & Babu (Science 210:330-2 1980 , gives rise in heterozygotes to mosaic patches of intestinal cells with altered autofluorescence under UV light. I have no idea about ease of scoring, but how many people have a UV light source on their dissecting scopes? (from JJTHADEN@life.uams.edu) lin-12 (n137) --only the heterozygous males mate, het hermaphrodites and homozygous do not, so that can be a problem later. The muv phenotype is fairly easy to score. (From Josh Kaplan via Garth Patterson patterson@frodo.mgh.harvard.edu) mec-4 --mec-4(e1611dm)X males mate.(from David Greenstein (David.Greenstein@mcmail.vanderbilt.edu) also from lmichel@lgme.u-strasbg.fr Michel Labouesse) rol-6 --For rol-6(su1006), I have used unintegrated transgenes, which are nice because they are easy to get rid of, and homo- or heterozygous integrated transgenes. The males mate fairly well in my hands.(from rhind@mendel.Berkeley.EDU ( Nick Rhind) sqt-3(sc63) --sqt-3(sc63)is a useful marker. It is temperature sensitive, homozygous Dpy, heterozygous Roller. Het males raised at the permissive temp mate fine. You'd have to then do your crosses at 25 and look for Roller cross progeny. (from meera sundaram, Han lab sundaram@beagle.Colorado.EDU>) unc-1 --We have had fairly good luck mating the dominant alleles of unc-1. They are coilers as males, and I will have to look back in my lab book from a couple years ago to see how many males we put on a plate. However, I know we were successful and it gives a phenotype easy to score. (from pgm2@po.CWRU.Edu (Philip G. Morgan) unc-22 --unc-22, the nicotine-induced "twitch" or vibration in a background of paralyzed wild type is supposed to be dominant. I like the twitch test but I've only tried it with homozygotes so far. unc-70(e524ts)V. --One marker I once used is unc-70(e524ts)V. The e524 males are very active in moving and mating well at 25C, the nonpermissive temperature, and e524/+ het is easy to tell by its curly movement at 25C. You can find it in the worm book(Wood, CSH)P551. Since e524 males mate more effectively at 15C(it looks wt) than 25C, I would keep the parents at 15C for mating, and transfer the eggs to 25C. (from Chenhui Wen, Iva Greenwald's lab, columbia university ) unc-108 (n501)I unc-103 (e1597)III unc-8 (n491)IV unc-70 (n493)V --Use heterozygous males of the following strains (dominant alleles of the uncs). These heterozygous males all mate, and it is easy to score cross- progeny as semi-uncs. (From: cori@itsa.ucsf.EDU (Cori Bargmann); also from "Leon Avery"