From owner-dros@hgmp.mrc.ac.uk Thu Oct 17 15:06:40 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id CE98D17A8A for ; Thu, 17 Oct 2002 15:06:37 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id EF8E217ACF for ; Thu, 17 Oct 2002 15:06:32 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 6729A17A8A; Thu, 17 Oct 2002 15:06:30 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 34B6617AD8 for ; Thu, 17 Oct 2002 13:56:39 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C1EE217A82; Thu, 17 Oct 2002 13:56:37 +0100 (BST) To: bionet-drosophila@net.bio.net From: Philippe.rouet@toulouse.inserm.fr (Philippe ROUET) Newsgroups: bionet.drosophila Subject: Schneider S2 cell culture Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <5.1.0.14.0.20021017150326.026350f0@pop.toulouse.inserm.fr> X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id A40E517AF5 for ; Thu, 17 Oct 2002 13:56:24 +0100 (BST) X-Received: from gw2-mail.cict.fr (gw2-mail.cict.fr [195.220.59.21]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 778F617A82 for ; Thu, 17 Oct 2002 13:56:18 +0100 (BST) X-Received: from gw2-mail.cict.fr (IDENT:root@localhost.localdomain [127.0.0.1]) by gw2-mail.cict.fr (8.12.5/8.12.5) with ESMTP id g9HCuEKm007623 for ; Thu, 17 Oct 2002 14:56:14 +0200 X-Received: from frouet.toulouse.inserm.fr (rech135-guesde.ups-tlse.fr [194.199.54.135]) by gw2-mail.cict.fr (8.12.5/8.12.5) with ESMTP id g9HCuEAT007597 for ; Thu, 17 Oct 2002 14:56:14 +0200 X-Sender: phirouet@pop.toulouse.inserm.fr X-Mailer: QUALCOMM Windows Eudora Version 5.1 X-To: dros@net.bio.net Date: Thu, 17 Oct 2002 15:06:30 +0100 (BST) X-Razor-id: 63a28d6239baa3e41268379f2e6f7929f0e5c076 Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi ! We are trying to culture Drosophila Schneider S2 cells and we do not have any experience with this cell line. Could you give us some tips such as how to separate the clamps for passaging and cell counting and how to transfect these cells ? With our best thanks, Best regards, Philippe Philippe ROUET, PhD CR1 INSERM Faculté de Médecine, INSERM U317 Service de Pharmacologie, 37, Allées Jules Guesde 31073 TOULOUSE FRANCE Tel : 00 33 (0)5 61 14 59 98 Fax : 00 33 (0)5 61 25 51 16 --- .