From owner-celegans@hgmp.mrc.ac.uk Fri Apr 28 15:20:42 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 93BE517A8D; Fri, 28 Apr 2000 15:20:41 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 6BEE117A61; Fri, 28 Apr 2000 15:20:40 +0100 (BST) To: celegans@net.bio.net Newsgroups: bionet.celegans Date: Thu, 27 Apr 2000 23:04:19 +0100 From: mmbarr.psi.sop@pharmacy.wisc.edu ("Maureen M. Barr") Subject: new lab setup: microscopes, etc. Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-Id: <20000428142040.6BEE117A61@mercury.hgmp.mrc.ac.uk> Sender: owner-celegans@hgmp.mrc.ac.uk Precedence: bulk I'm getting ready to purchase alot of new equipment for a new lab. What I would like is your experience and suggestions regarding standard C. elegans lab mainstays... Dissecting scopes: Zeiss Stemi 2000 vs. Leica vs. Nikon vs. any others. Injection set ups: Eppendorf vs. Naroshege vs. competitors Micropipette pullers: Sutter P-87 vs. competitors Plate pourers: ???? Leica/Wild Makroscope M420: does anything compare???? I grew up with Zeiss Axiovert, Axioplan, and Axioscop scopes and am considering purchasing their offspring (Axiovert S100, Axioscop 2 and Axioplan 2I). The scopes will be used for DIC, fluorescence, ablation, injection, etc... If Zeiss wasn't so much more expensive than the others (such as Nikon), it would be a done deal. Now I'm considering and comparing comparable scopes. Does anyone have any words of wisdom? I'll save the questions on digital cameras for another time. I will gladly compile all responses into a spread sheet for future reference. Thanks! Maureen Barr ******************************* Maureen M. Barr University of Wisconsin-Madison School of Pharmacy 425 North Charter St. Madison, WI 53706 Office: 5256 Chamberlin Hall Phone: 608-265-1174 Fax: 608-262-3397 ******************************* --- .