From owner-cellbiol@net.bio.net Mon Feb 03 22:00:00 1997
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From: "Dr E. Buxbaum" <EB15@le.ac.uk>
Newsgroups: bionet.cellbiol
Subject: Re: glycosylation
Date: 4 Feb 1997 17:40:41 GMT
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delgadoi@PILOT.MSU.EDU ("Ivan J Delgado") wrote:
>
> A question regarding glycosylation of proteins and how that looks in 
> a western.

Glycosylation leads to spread out, unsharp bands in SDS-PAGE (and 
consequentially in Westerns), which have a higher molecular weight than 
the unglycosylated protein. 

A nice way to check for glycosylation is to stain the gel or the western 
blot with Feulgen's reagent. Gives bright red bands, a very beautifull 
color.


.