From owner-drosophila@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!PSUHMED.RCF.HMC.PSU.EDU!jlee From: jlee@PSUHMED.RCF.HMC.PSU.EDU (Jae-Seong Lee) Newsgroups: bionet.drosophila Subject: Post-doctoral Position Date: 9 Oct 1995 18:11:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 174 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510100103.AA22593@sys-a> NNTP-Posting-Host: net.bio.net I would be highly pleased to introduce myself to you as a post-doctoal fellow in Department of Pathology, College of Medicine, The Pennsylvania State University. I am deeply interested in your field and so I strongly hope I could join in your lab as a post-doctoral fellow. In 1982, I entered in Department of Biology, Hanynag University, Seoul in Korea and learned the extensive basic biology for 4 years until 1986. After graduate, I enrolled in the Department of Biology (major in Cell Biology), the graduate school of Hayang University and I had worked in the field of the cellular toxicology using fish cell line, ULF-23HU (Umbra limi), and also had the responsibility of husbandry of 1 aquarium for caring about the hermaphroditic fish, Rivulus marmoratus. At that course, I learned the basic cellular toxicology (cytotoxicity testing, cytigenetic studies on sister chromatid exchanges and chromosome aberration testing), fish cell culture (characterization of cell line including the measurement of cell cycle using autoradiography and cytogentic analysis). My master works were published in In Vitro Cell. Dev. Biol., 25: 987-994 (1989), the abstract of International Icthyological Congress (The Hague, The Netherland; 1992), and Mutation Research, 268: 239-246 (1992). For 1 and half year (Sept. 1988-Feb. 1990), I served in the Army of Korea because this is the obligation of all men of Korea. In March 1990, I enrolled in the Department of Biology (major in Molecular Biology) of Hanyang University Graduate School. I worked on primary cell culture of the hermaphroditic fish, R. marmoratus for establishment of Rivulus cell line by August 1991. Then, I established the method of Rivulus cell culture, and prepared the Rivulus chromosomes for cytogenetic study. After September 1991, I moved to "Lab of Molecular Virology (Boss; Prof. Joonho Choe)", Department of Biological Science, Korea Advanced Institute of Science and Technology (KAIST) that is the most famous leading University in Korea. In there, I carried out my Ph.D. work elucidating out on the structure of ras genes in the hermaphroditic fish, R. marmoratus. At first, I made the Rivulus genomic DNA library for isolating of intact ras genes. I had cloned 5 kinds of different ras genes. Lately, I nominated them as a Rivulus Ha-, Ki-, R-ras gene, respectively. Two clones did not analyze yet. Rivulus Ha- and Ki-ras gene were published in two papers in "Biochem. Mol. Biol. Int." because they showed totally different characteristics in structure as compared in those of mammals. Now I am writing paper of Rivulus R-ras gene for submitting to "Gene". As you may know, R-ras gene is recently interested in the role of anti-oncogene and signal transduction in cell. Besides on these ras genes, I had cloned at least 3 different kinds of Rivulus heat shock protein 70 genes, and DNA repair gene (O6-alkylguanine methyltransferase gene). O6-MT gene is inducible gene, and involved the occurence of tumor by alkylating agents. Meanwhile, out team (leader; Prof. Eun-Ho Park) was interested in carcinogenecity of alkylating agents in R. marmoratus. So, we published our data on tumor incidence by alkylating agents in "Japanese Journal of Cancer Research". For doing more detail analysis, I analyzed the point mutation of Rivulus Ha- and Ki-ras gene exon 1 and 2 of alkylating agent-induced thyroid tumor in R. marmoratus. These data were not published yet. In March 1994, I returned to Hanynag University for writing my thesis. On October 19 last year, I joined in here for extending my experiences in fish. Now I isolated many genes in zebrafish. My contract would be expired by November 15, 1995. So, I need to move another lab. Thanks. My C.V. is as follow, --------- Personnel --------- Date of Birth; Sept. 28, 1963 Nationality: South Korea Sex: Male Marital Status: Married in 1994 (Spouse: Joo-Ok Yoon; 1 child) Home: 15 Univeristy Manor East Hershey, PA 17033 Tel & Fax: (717) 531-8447 Lab: Department of Pathology College of Medicine The Pennsylvania State University Hershey, PA 17033 Tel. (717) 531-4704 e-mail: jlee@cor-mail.biochem.hmc.psu.edu ----------- Education ----------- 1982-1986 Department of Biology, Hanyang Unviersity, Seoul, Korea. B.S. 1986-1988 Department of Biology, Hanyang Unviersity Graduate School, Seoul Korea. M.S. Supervisor: Prof. Eun-Ho Park 1990-1994 Department of Biology, Hanyang Unviersity Graduate School, Seoul Korea. Ph.D. Supervisor: Prof. Eun-Ho Park ------------ Experiences ------------ 1988-1990 Military Service, The Army of Korea 1990-1991 Research Assistant, Research Institute of Environmental Sciences, Hayang University, Seoul, Korea 1991-1992 Research and Teaching Assistant, Dept. of Biology, Hanyang University, Seoul, Korea 1994-Present Post-doctoral Fellow, Dept. of Pathology, College of Medicine The Pennsylvania State University, Hershey, Pennsylvania Supervisor: Asst. Prof. Keith C. Cheng -------------------- List of publications -------------------- 1. Lee, J.-S., 1988. Growth kinetics and cytogenetic characteristics of the fish cell line, ULF (Umbra limi fin)-23HU. M.S. dissertation, Hanyang Univeristy, Seoul, Korea. pp. 1-34. 2. Park, E.-H., J.-S. Lee, A.-K. Yi and H. Etoh, 1989. Fish cell line (ULF-23HU) derived from the fin of the central mudminow (Umbra limi): suitable characterstics for clastogenecity assay. In Vitro Cell. Dev. Biol., 25: 987-994. (USA) 3. Park, E.-H., Y. J. Kim, D. H. Byun, J.-Y. Lee and J.-S. Lee, 1992. baseline frequency of sister chromatid exchanges in 142 persons of the general Korean population. Mutat. Res., 268: 239-246. (The Netherlands) 4. Lee, J.-S., 1994. Structure of the ras oncogenes in the hermaphrodic fish, Rivulus marmoratus. Ph.D. dissertation, Hanyang Unviversity, Seoul, Korea. pp. 1-104. 5. Lee, J.-S., J. Choe and E.-H. Park, 1994. Absence of the intron-D-exon of c-Ha-ras oncogene in the hermaphrodic fish Rivulus marmoratus (Teleostei: Rivulidae). Biochem. Mol. Biol. Int., 34: 921-926. (Australia) 6. Lee, J.-S., J. Choe and E.-H. Park, 1995. Genomic structure of the c-Ki-ras proto-oncogene of the hermaphroditic fish Rivulus marmoratus (Teleostei: Rivulidae). Biochem. Mol. Biol. Int., 35: 57-63. (Australia) 7. Lee, J.-S., J. Choe and E.-H. Park, 1995. Isolation and characterization of R-ras gene in the hermaphroditic fish Rivulus marmoratus. In Preparation. ----------- References ----------- 1. Prof. Eun-Ho Park Department of Biology College of Natural Sciences Hanyang University Seoul 133-791, South Korea Tel. +82-2-290-0953 Fax +82-2-299-3495 2. Asst. Prof. Keith C. Cheng Department of Pathology College of Medicine The Pennsylvania State University Hershey, PA 17033 Tel. (717) 531-5635 Fax (717) 531-5021 3. Asso. Prof. Joonho Choe Department of Biological Science Korea Advanced Institute of Science and Technology Taejon 305-701, South Korea Tel. +82-42-869-4020 Fax +82-42-869-4010 Thank you for your consideration. Best wishes, Jae-Seong Lee, Ph.D. Department of Pathology College of Medicine The Pennsylvania State University Hershey, PA 17033 .