From owner-drosophila@net.bio.net Sat Nov 19 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!spool.mu.edu!uwm.edu!news.doit.wisc.edu!f181-169.net.wisc.edu!user From: wrengels@facstaff.wisc.edu (Bill Engels) Newsgroups: bionet.drosophila Subject: Re: deletion mapping by PCR? Date: Sat, 19 Nov 1994 23:17:18 -0600 Organization: University of Wisconsin Lines: 25 Message-ID: References: <3ab9s7$pn4@bigfoot.wustl.edu> NNTP-Posting-Host: f181-169.net.wisc.edu In article <3ab9s7$pn4@bigfoot.wustl.edu>, shaffer@biodec (Christopher Shaffer) wrote: >The > idea is to use a suitable embryonic marker which would allow one to > collect embryo's homozygous for the deletion chromosome. These embryos > could then be used in a PCR based assay looking for the presence of the > gene. If anyone has tried this technique I would appreciate any > pointers. 19 November 1994 Yes, Carlos Flores in my lab did something like that. Except that it was easier because we knew the deletion homozygotes lived to the pupal stage. He used the Tubby marker to pick out pupae that had the deletions and PCR¹d them with primers suspected of being within the deletion along with some positive control primers. It worked fine. \\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \ William R. Engels : WREngels@facstaff.wisc.edu Genetics Department, 445 Henry Mall : office: (608) 263-2213 University of Wisconsin : lab: (608) 262-5578 Madison, WI 53706 : FAX: (608) 262-2976 ---------------------------------------------------------------------- .