From owner-celegans@net.bio.net Mon Mar 25 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!yama.mcc.ac.uk!zippy.dct.ac.uk!str-ccsun!udcf.gla.ac.uk!news Newsgroups: bionet.celegans Subject: markers for transformation Message-ID: From: gbga14@ucdf.gla.ac.uk Date: Tue, 26 Mar 1996 13:37:11 GMT Sender: news@udcf.gla.ac.uk (News) Organization: Glasgow University Computing Service Lines: 9 Can anyone suggest an unc that has been cloned that would be good to use as a marker (by rescue) for constructing free arrays. Idealy I want an unc that can be scored during most stages and in a double background with a relatively strong dpy. Obviously it would also be good if the wild type cloned gene was relatively small and available on a plasmid rather than a cosmid. One final requirement - temperature sensitivity would prove problematic. (One that sings, dances and pours petri dishes would also be good!) .