From owner-cellbiol@net.bio.net Fri Feb 28 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!newspump.sol.net!howland.erols.net!swrinde!news.uh.edu!news.uth.tmc.edu!not-for-mail From: Debdutta Bandyopadhyay Newsgroups: bionet.cellbiol Subject: Looking for anti ubiquitin and anti p95 antibodies Date: Fri, 28 Feb 1997 14:12:00 -0600 Organization: UTMDACC Lines: 11 Message-ID: <33173C10.3F92@utmdacc.mda.uth.tmc.edu> Reply-To: dbandyop@utmdacc.mda.uth.tmc.edu NNTP-Posting-Host: cgr43.mdacc.tmc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (Win95; I) Hi friends, I am looking for some good anti-ubiquitin antibody and a protocol for this. Specially I have an idea that Ubiquitinated proteins can not be detected unless you autoclave the nitrocellulose after the transfer and befor blocking. Also I am interested in finding out a good antibody for p95 (vav) oncoprotein. If any one can come up with any suggestion I will really be helpful. Thanks; Debdutta Bandyopadhyay .