From owner-drosophila@net.bio.net Wed Sep 13 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!usc!news.cerf.net!newsserver.sdsc.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newsstand.cit.cornell.edu!NewsWatcher!user From: sg32@postoffice.mail.cornell.edu (Susan Glueck) Newsgroups: bionet.drosophila Subject: genome size and library screening Date: 14 Sep 1995 14:44:44 GMT Organization: Cornell University Lines: 10 Sender: sg32@cornell.edu (Verified) Message-ID: NNTP-Posting-Host: 132 The "ballpark" in our lab for screening genomic libraries of D. melanogaster is to screen 1 x 10^6 plaques (one million) in order to get a fair representative of the genome. What kind of numbers do you employ? Furthemore, how much larger than Drosophila melanogaster is the genome of Musca domestica? Then how many plaques would it be necessary to screen to obtain a fair representation of that genome? Susan Glueck sg32@cornell.edu .